p3A2-Flag
(Plasmid
#234803)
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PurposeMammalian expression of bovine arrestin-2 with C-terminal Flag tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234803 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameArrestin-2
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SpeciesB. taurus (bovine)
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Mutationnone
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Entrez GeneARRB2
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Tag
/ Fusion Protein
- Flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3A2-Flag was a gift from Vsevolod Gurevich (Addgene plasmid # 234803 ; http://n2t.net/addgene:234803 ; RRID:Addgene_234803) -
For your References section:
Ubiquitin ligase parkin promotes Mdm2-arrestin interaction but inhibits arrestin ubiquitination. Ahmed MR, Zhan X, Song X, Kook S, Gurevich VV, Gurevich EV. Biochemistry. 2011 May 10;50(18):3749-63. doi: 10.1021/bi200175q. Epub 2011 Apr 12. 10.1021/bi200175q PubMed 21466165