pVEA3-DR
(Plasmid
#234800)
-
PurposeMammalian expression of bovine arrestin-3 (D291R)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 234800 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA3
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameArrestin-3
-
SpeciesB. taurus (bovine)
-
MutationD291R
-
Entrez GeneARR3
-
Tag
/ Fusion Protein
- Venus (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pVEA3-DR was a gift from Vsevolod Gurevich (Addgene plasmid # 234800 ; http://n2t.net/addgene:234800 ; RRID:Addgene_234800) -
For your References section:
GPCR Binding and JNK3 Activation by Arrestin-3 Have Different Structural Requirements. Zheng C, Weinstein LD, Nguyen KK, Grewal A, Gurevich EV, Gurevich VV. Cells. 2023 Jun 6;12(12):1563. doi: 10.3390/cells12121563. 10.3390/cells12121563 PubMed 37371033