p3A2-RE
(Plasmid
#234794)
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PurposeMammalian expression of bovine arrestin-2 (R169E)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234794 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameArrestin-2
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SpeciesB. taurus (bovine)
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MutationR169E
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Entrez GeneARRB2
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3A2-RE was a gift from Vsevolod Gurevich (Addgene plasmid # 234794 ; http://n2t.net/addgene:234794 ; RRID:Addgene_234794) -
For your References section:
The nature of the arrestin x receptor complex determines the ultimate fate of the internalized receptor. Pan L, Gurevich EV, Gurevich VV. J Biol Chem. 2003 Mar 28;278(13):11623-32. doi: 10.1074/jbc.M209532200. Epub 2003 Jan 13. 10.1074/jbc.M209532200 PubMed 12525498