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pcDNA3.1-PDGFRA-V561D-V5/HIS
(Plasmid #234762)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234762 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1-V5/His
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PDGFRA-V561D receptor tyrosine kinase, full length
  • Species
    H. sapiens (human)
  • Mutation
    V561D substitution
  • Entrez Gene
    PDGFRA (a.k.a. CD140A, PDGFR-2, PDGFR2)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5/His (C terminal on insert)

Cloning Information

  • Cloning method TOPO Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-PDGFRA-V561D-V5/HIS was a gift from Pavel Krejčí (Addgene plasmid # 234762 ; http://n2t.net/addgene:234762 ; RRID:Addgene_234762)

  • For your References section:

    Inhibitor repurposing reveals ALK, LTK, FGFR, RET and TRK kinases as the targets of AZD1480. Gudernova I, Balek L, Varecha M, Kucerova JF, Kunova Bosakova M, Fafilek B, Palusova V, Uldrijan S, Trantirek L, Krejci P. Oncotarget. 2017 Nov 27;8(65):109319-109331. doi: 10.18632/oncotarget.22674. eCollection 2017 Dec 12. 10.18632/oncotarget.22674 PubMed 29312610
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