Venus-155-239-Gbeta1
(Plasmid
#234751)
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PurposeMammalian expression of BiFC Venus-betagamma (with Venus-1-155-Ggamma2)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234751 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGNB1
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SpeciesH. sapiens (human)
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Entrez GeneGNB1 (a.k.a. HG2A, MDS, MRD42)
- Promoter CMV
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Tag
/ Fusion Protein
- VN155 (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Venus-155-239-Gbeta1 was a gift from Nevin Lambert (Addgene plasmid # 234751 ; http://n2t.net/addgene:234751 ; RRID:Addgene_234751) -
For your References section:
The c-terminus of GRK3 indicates rapid dissociation of G protein heterotrimers. Hollins B, Kuravi S, Digby GJ, Lambert NA. Cell Signal. 2009 Jun;21(6):1015-21. doi: 10.1016/j.cellsig.2009.02.017. Epub 2009 Mar 1. 10.1016/j.cellsig.2009.02.017 PubMed 19258039