CMV-LgBit
(Plasmid
#234738)
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PurposeMammalian expression of large NanoBit fragment (LgBit)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234738 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLgBit
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SpeciesSynthetic
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV-LgBit was a gift from Nevin Lambert (Addgene plasmid # 234738 ; http://n2t.net/addgene:234738 ; RRID:Addgene_234738) -
For your References section:
Visualization of endogenous G proteins on endosomes and other organelles. Jang W, Senarath K, Feinberg G, Lu S, Lambert NA. Elife. 2024 Nov 8;13:RP97033. doi: 10.7554/eLife.97033. 10.7554/eLife.97033 PubMed 39514269