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Addgene

pCAG-eCas9-T2A-EGFP-U6-gRNA-no ITR and f1 ori
(Plasmid #234724)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234724 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG-eCas9-GFP-U6-gRNA
  • Backbone manufacturer
    Jizhong Zou, Addgene plasmid #79145
  • Backbone size (bp) 9413
  • Modifications to backbone
    The AAV2 ITR and f1 ori elements were removed.
  • Vector type
    Mammalian Expression, CRISPR
  • Promoter CAG promoter
  • Tag / Fusion Protein
    • T2A-GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-eCas9-T2A-EGFP-U6-gRNA-no ITR and f1 ori was a gift from Kirill Martemyanov (Addgene plasmid # 234724 ; http://n2t.net/addgene:234724 ; RRID:Addgene_234724)

  • For your References section:

    Efficient in vivo labeling of endogenous proteins with SMART delineates retina cellular and synaptic organization. Zhao C, Cao Y, Ibrahim N, Wang Y, Martemyanov KA. Nature Communications 2025 10.1038/s41467-025-58945-6
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