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Addgene

pTcTurboID-GW_GFP-NLS
(Plasmid #234716)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234716 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTcTurboID-GW
  • Vector type
    Trypanosomatid expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP-3xNLS
  • Species
    Other
  • Promoter T7
  • Tag / Fusion Protein
    • 3xHA (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer ataaacagggagccctgc
  • 3′ sequencing primer CAAGGACAGAAAACGGTAAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Nuclear localization signal of SV40 (3x)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTcTurboID-GW_GFP-NLS was a gift from Esteban Serra (Addgene plasmid # 234716 ; http://n2t.net/addgene:234716 ; RRID:Addgene_234716)
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