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Addgene

pAAV-Esyn-FlpX-TVA950-EYFP-WPRE
(Plasmid #234710)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234710 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV eSyn FlpX
  • Backbone size w/o insert (bp) 4778
  • Total vector size (bp) 6164
  • Vector type
    Mammalian Expression, AAV ; has fDIO cassette for Flp-dependent expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TVA950-eYFP
  • Species
    Synthetic; Coturnix japonica and synthetic
  • Insert Size (bp)
    1224
  • GenBank ID
    OR551243.1
  • Promoter enhanced Synapsin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer gcacgggcgcgaccatctgc
  • 3′ sequencing primer gtaatccagaggttgattatcgataagctg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.08.28.610136 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-Esyn-FlpX-TVA950-EYFP-WPRE was a gift from David Ng (Addgene plasmid # 234710 ; http://n2t.net/addgene:234710 ; RRID:Addgene_234710)

  • For your References section:

    Segregated basal ganglia output pathways correspond to genetically divergent neuronal subclasses. Mendelsohn AI, Nikoobakht L, Bikoff JB, Costa RM. bioRxiv [Preprint]. 2024 Sep 18:2024.08.28.610136. doi: 10.1101/2024.08.28.610136. 10.1101/2024.08.28.610136 PubMed 39257765
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