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Addgene

pET28a-SET
(Plasmid #234708)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234708 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a(+)
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6130
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SET
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2455
  • Entrez Gene
    SET (a.k.a. 2PP2A, I2PP2A, IGAAD, IPP2A2, MRD58, PHAPII, TAF-I, TAF-IBETA)
  • Promoter lacI
  • Tag / Fusion Protein
    • (His)6 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    PCR

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-SET was a gift from Lienhard Schmitz (Addgene plasmid # 234708 ; http://n2t.net/addgene:234708 ; RRID:Addgene_234708)

  • For your References section:

    CDK1-mediated phosphorylation at H2B serine 6 is required for mitotic chromosome segregation. Seibert M, Kruger M, Watson NA, Sen O, Daum JR, Slotman JA, Braun T, Houtsmuller AB, Gorbsky GJ, Jacob R, Kracht M, Higgins JMG, Schmitz ML. J Cell Biol. 2019 Apr 1;218(4):1164-1181. doi: 10.1083/jcb.201806057. Epub 2019 Feb 14. 10.1083/jcb.201806057 PubMed 30765437
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