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Addgene

GFP-SET(1-218)
(Plasmid #234707)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234707 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcGFP-C1
  • Backbone size w/o insert (bp) 4731
  • Total vector size (bp) 5342
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SET
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    682
  • Mutation
    expresses only the N-terminal SET part (1-218)
  • Entrez Gene
    SET (a.k.a. 2PP2A, I2PP2A, IGAAD, IPP2A2, MRD58, PHAPII, TAF-I, TAF-IBETA)
  • Promoter CMV
  • Tag / Fusion Protein
    • AcGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpm2I (not destroyed)
  • 3′ cloning site BclI (not destroyed)
  • 5′ sequencing primer AcGFP1
  • 3′ sequencing primer SV40 poly A reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    PCR

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-SET(1-218) was a gift from Lienhard Schmitz (Addgene plasmid # 234707 ; http://n2t.net/addgene:234707 ; RRID:Addgene_234707)

  • For your References section:

    CDK1-mediated phosphorylation at H2B serine 6 is required for mitotic chromosome segregation. Seibert M, Kruger M, Watson NA, Sen O, Daum JR, Slotman JA, Braun T, Houtsmuller AB, Gorbsky GJ, Jacob R, Kracht M, Higgins JMG, Schmitz ML. J Cell Biol. 2019 Apr 1;218(4):1164-1181. doi: 10.1083/jcb.201806057. Epub 2019 Feb 14. 10.1083/jcb.201806057 PubMed 30765437
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