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Addgene

GFP-LacI-Cyclin B1 ND
(Plasmid #234706)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234706 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    GFP-LacI-polylinker
  • Backbone size w/o insert (bp) 7307
  • Total vector size (bp) 8586
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cyclin B1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Entrez Gene
    CCNB1 (a.k.a. CCNB)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer EGFP
  • 3′ sequencing primer bGH polyA reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-LacI-Cyclin B1 ND was a gift from Lienhard Schmitz (Addgene plasmid # 234706 ; http://n2t.net/addgene:234706 ; RRID:Addgene_234706)

  • For your References section:

    CDK1-mediated phosphorylation at H2B serine 6 is required for mitotic chromosome segregation. Seibert M, Kruger M, Watson NA, Sen O, Daum JR, Slotman JA, Braun T, Houtsmuller AB, Gorbsky GJ, Jacob R, Kracht M, Higgins JMG, Schmitz ML. J Cell Biol. 2019 Apr 1;218(4):1164-1181. doi: 10.1083/jcb.201806057. Epub 2019 Feb 14. 10.1083/jcb.201806057 PubMed 30765437
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