p357_DG
(Plasmid
#234700)
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Purpose(Empty Backbone) Golden gate domesticated pET28b for scarless cloning with His10-GST-3C
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 234700 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28b
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Vector typeBacterial Expression
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Tag
/ Fusion Protein
- His10-GST-3C (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
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MutationNo
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Tag
/ Fusion Protein
- His10-GST-3C (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The construct features an N-terminal His10-GST fusion followed by a 3C protease cleavage site. There is no glycine scar at the C-terminus. BsaI recognition sites flank mScarlet3 to enable easy visualization of successful cloning through loss of red color. 5' Cloning Site: BsaI (destroyed), 3' Cloning Site: BsaI (destroyed).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p357_DG was a gift from Matthew Wohlever (Addgene plasmid # 234700 ; http://n2t.net/addgene:234700 ; RRID:Addgene_234700) -
For your References section:
A suite of pre-assembled, pET28b-based Golden Gate vectors for efficient protein engineering and expression. Gaur D, Wohlever ML. Protein Sci. 2025 Apr;34(4):e70106. doi: 10.1002/pro.70106. 10.1002/pro.70106 PubMed 40130802
