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hnRNPA1_allW_D262V-FLAG-IRES-eGFP
(Plasmid #234621)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234621 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4/TO/myc-His A
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hnRNPA1 allW D262V
  • Species
    H. sapiens (human)
  • Mutation
    Famillial ALS mutation D262V, all aromatic amino acids in C-terminal domain (186-320) mutated to W, except for two Y-s in the hexapeptide fibril core (SYNVFG) and PY-NLS
  • Entrez Gene
    HNRNPA1 (a.k.a. ALS19, ALS20, HNRPA1, HNRPA1L3, IBMPFD3, MPD3, UP 1, hnRNP A1, hnRNP-A1)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.02.28.582569 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hnRNPA1_allW_D262V-FLAG-IRES-eGFP was a gift from Tanja Mittag (Addgene plasmid # 234621 ; http://n2t.net/addgene:234621 ; RRID:Addgene_234621)

  • For your References section:

    Metastable condensates suppress conversion to amyloid fibrils. Das T, Zaidi F, Farag M, Ruff KM, Messing J, Taylor JP, Pappu RV, Mittag T. bioRxiv [Preprint]. 2024 Mar 3:2024.02.28.582569. doi: 10.1101/2024.02.28.582569. 10.1101/2024.02.28.582569 PubMed 38464104
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