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Addgene

A1-LCD 5W D262V
(Plasmid #234617)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234617 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDest17
  • Backbone manufacturer
    Invtirogen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing lab recommends BL21-Gold (DE3) E. coli for protein expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    hnRNPA1_LCD_5W D262V
  • Alt name
    A1-LCD 5W D262V
  • Species
    H. sapiens (human)
  • Mutation
    F222W, F228W, Y237W, D262V, Y305W, F320W
  • Entrez Gene
    HNRNPA1 (a.k.a. ALS19, ALS20, HNRPA1, HNRPA1L3, IBMPFD3, MPD3, UP 1, hnRNP A1, hnRNP-A1)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert created through codon optimized gene synthesis. TEV cleavage leaves N-terminal GS tag.

Please visit https://doi.org/10.1101/2024.02.28.582569 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    A1-LCD 5W D262V was a gift from Tanja Mittag (Addgene plasmid # 234617 ; http://n2t.net/addgene:234617 ; RRID:Addgene_234617)

  • For your References section:

    Metastable condensates suppress conversion to amyloid fibrils. Das T, Zaidi F, Farag M, Ruff KM, Messing J, Taylor JP, Pappu RV, Mittag T. bioRxiv [Preprint]. 2024 Mar 3:2024.02.28.582569. doi: 10.1101/2024.02.28.582569. 10.1101/2024.02.28.582569 PubMed 38464104
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