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Addgene

A1-LCD D262N
(Plasmid #234608)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234608 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDest17
  • Backbone manufacturer
    Invtirogen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing lab recommends BL21-CodonPlus(DE3) RIPL E. coli for protein expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    hnRNPA1_LCD_D262N
  • Alt name
    A1-LCD D262N
  • Species
    H. sapiens (human)
  • Mutation
    C-terminal domain (186-320) bearing familial ALS mutation D262N
  • Entrez Gene
    HNRNPA1 (a.k.a. ALS19, ALS20, HNRPA1, HNRPA1L3, IBMPFD3, MPD3, UP 1, hnRNP A1, hnRNP-A1)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Not codon optimized for bacterial expression. TEV cleavage leaves N-terminal GS tag.

Please visit https://doi.org/10.1101/2024.02.28.582569 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    A1-LCD D262N was a gift from Tanja Mittag (Addgene plasmid # 234608 ; http://n2t.net/addgene:234608 ; RRID:Addgene_234608)

  • For your References section:

    Metastable condensates suppress conversion to amyloid fibrils. Das T, Zaidi F, Farag M, Ruff KM, Messing J, Taylor JP, Pappu RV, Mittag T. bioRxiv [Preprint]. 2024 Mar 3:2024.02.28.582569. doi: 10.1101/2024.02.28.582569. 10.1101/2024.02.28.582569 PubMed 38464104
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