pJPC432
(Plasmid
#234528)
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PurposeFLP Recombinase switch; plant T-DNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234528 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTRANS_200d
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRUBY
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Alt namenptII
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SpeciesSugar beet, E. coli
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Mutationnone
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJPC432 was a gift from Daniel Voytas (Addgene plasmid # 234528 ; http://n2t.net/addgene:234528 ; RRID:Addgene_234528) -
For your References section:
Viral delivery of recombinases activates heritable genetic switches in plants. Chamness JC, Cody JP, Cruz AJ, Voytas DF. Plant Physiol. 2025 Mar 1;197(3):kiaf073. doi: 10.1093/plphys/kiaf073. Epub 2025 Feb 21. 10.1093/plphys/kiaf073 PubMed 40111273