pDONR223-GNE
(Plasmid #23448)

• Depositing Labs: William Hahn, David Root
• Publication: Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627

Backbone

• Vector backbone: pDONR223
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5005
• Vector type: Gateway Donor vector

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GNE
• Alt name: glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
• Species: H. sapiens (human)
• Insert Size (bp): 2169
• Mutation: D407G
• Entrez Gene: GNE (a.k.a. DMRV, GLCNE, IBM2, NM, Uae1)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13pUC-fwd
• 3′ sequencing primer: M13rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.

A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.