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pT3TS-Hf-RfxCas13d-HA
(Plasmid #234479)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234479 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT3TS
  • Total vector size (bp) 6115
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RfxCas13d
  • Alt name
    CasRx
  • Species
    Ruminococcus flavefaciens XPD3002
  • Insert Size (bp)
    2934
  • Mutation
    Changed four alanine to valine in positions 134, 140, 141 and 143 (A134V, A140V, A141V and A143V)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmid #141320 used as template

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Point mutations performed as described in Tong et al., 2023 (Nat Bio) to generate a High-Fidelity version of RfxCas13d (Hf-RfxCas13d).

Please visit https://doi.org/10.1101/2024.10.08.617220 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT3TS-Hf-RfxCas13d-HA was a gift from Miguel Angel Moreno-Mateos (Addgene plasmid # 234479 ; http://n2t.net/addgene:234479 ; RRID:Addgene_234479)

  • For your References section:

    Enhanced RNA-targeting CRISPR-Cas technology in zebrafish. Moreno-Sanchez I, Hernandez-Huertas L, Nahon-Cano D, Martinez-Garcia PM, Treichel AJ, Gomez-Marin C, Tomas-Gallardo L, da Silva Pescador G, Kushawah G, Egidy R, Perera A, Diaz-Moscoso A, Cano-Ruiz A, Walker JA 2nd, Munoz MJ, Holden K, Galceran J, Nieto MA, Bazzini AA, Moreno-Mateos MA. Nat Commun. 2025 Mar 16;16(1):2591. doi: 10.1038/s41467-025-57792-9. 10.1038/s41467-025-57792-9 PubMed 40091120
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