pAG Retro EGFP Neo
(Plasmid
#234464)
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PurposeControl retroviral vector expressing Green Fluorescent Protein. Neomycin selection.
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234464 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepAG retro
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Backbone manufacturerAddgene
- Backbone size w/o insert (bp) 6222
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Vector typeRetroviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGreen Fluorescent Protein
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SpeciesSynthetic
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Insert Size (bp)728
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SnaBI (not destroyed)
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer pBABE 3' (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG Retro EGFP Neo was a gift from Melina Fan & Addgene Research Program (Addgene plasmid # 234464 ; http://n2t.net/addgene:234464 ; RRID:Addgene_234464)