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Addgene

pAG Retro EGFP Puro
(Plasmid #234463)

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Item Catalog # Description Quantity Price (USD)
Plasmid 234463 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pAG retro
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 6027
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Green Fluorescent Protein
  • Species
    Synthetic
  • Insert Size (bp)
    728

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SnaBI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG Retro EGFP Puro was a gift from Melina Fan & Addgene Research Program (Addgene plasmid # 234463 ; http://n2t.net/addgene:234463 ; RRID:Addgene_234463)
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