pAG Retro Puro Empty
(Plasmid
#234458)
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Purpose(Empty Backbone) Retroviral vector for cloning and expressing your gene of interest. Puromycin selection.
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Depositing Labs
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 234458 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepAG retro
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Backbone manufacturerAddgene
- Backbone size (bp) 6027
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Vector typeRetroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer pBABE 3' (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG Retro Puro Empty was a gift from Melina Fan & Addgene Research Program (Addgene plasmid # 234458 ; http://n2t.net/addgene:234458 ; RRID:Addgene_234458)
Map uploaded by the depositor.
Map uploaded by the depositor.
