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pEN017-SpCas9-hiNLS-t-M4
(Plasmid #234455)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234455 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEC-K-MBP
  • Backbone manufacturer
    pET-based custom vector
  • Backbone size w/o insert (bp) 4934
  • Total vector size (bp) 9138
  • Modifications to backbone
    HA and MBP removed
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9 hiNLS t-M4
  • Alt name
    SpCas9 0C triNLS hiNLS M4
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4204
  • Mutation
    Cys at positions 80 and 574 have been mutated to Ser; Met at position 1 has been mutated to Ser; inserted hiNLS c-Myc (t-M4, hairpin internal)
  • Tags / Fusion Proteins
    • His6 (N terminal on backbone)
    • c-Myc (N terminal on insert)
    • SV40 (C terminal on insert)
    • Nucleoplasmin (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEN017-SpCas9-hiNLS-t-M4 was a gift from Ross Wilson (Addgene plasmid # 234455 ; http://n2t.net/addgene:234455 ; RRID:Addgene_234455)
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