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Addgene

pAAV-syn-iGluSnFR4f-NGR-WPRE
(Plasmid #234438)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234438 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-Syn
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Depositing lab recommends limiting overnight liquid culture to 16 h at higher temperatures (37 C) to maintain ITR integrity, rather than 30 C for 20 h.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iGluSnFR4f-NGR
  • Alt name
    variant 9601
  • Species
    Synthetic; E. coli, A. victoria (jellyfish)
  • Insert Size (bp)
    1791
  • Promoter Synapsin
  • Tags / Fusion Proteins
    • IgK chain (N terminal on insert)
    • Myc epi tag-NGR GPI anchor (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-syn-iGluSnFR4f-NGR-WPRE was a gift from GENIE Project (Addgene plasmid # 234438 ; http://n2t.net/addgene:234438 ; RRID:Addgene_234438)

  • For your References section:

    Glutamate indicators with increased sensitivity and tailored deactivation rates. Aggarwal A, Negrean A, Chen Y, Iyer R, Reep D, Liu A, Palutla A, Xie ME, MacLennan BJ, Hagihara KM, Kinsey LW, Sun JL, Yao P, Zheng J, Tsang A, Tsegaye G, Zhang Y, Patel RH, Arthur BJ, Hiblot J, Leippe P, Tarnawski M, Marvin JS, Vevea JD, Turaga SC, Tebo AG, Carandini M, Rossi LF, Kleinfeld D, Konnerth A, Svoboda K, Turner GC, Hasseman J, Podgorski K. bioRxiv 2025.03.20.643984 10.1101/2025.03.20.643984
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