Skip to main content
Addgene

pCRISPomyces-2BD
(Plasmid #234428)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234428 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCRISPomyces-2
  • Backbone size w/o insert (bp) 7510
  • Total vector size (bp) 11707
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Modified Cas9 with polyaspartate linking
  • Alt name
    Cas9-BD
  • Species
    Synthetic
  • Insert Size (bp)
    4197
  • Mutation
    Linked polyaspartate using glycine-serine linker

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGT AGG CCG ACA GGC TCA TG
  • 3′ sequencing primer TTC CAG GGG GAA ACG CCT GG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor notes that there are SNPs in pSG5 Rep that cause the following amino acid substitutions: L468H R467Q C354W S215A K165E H144R, but these are not of concern for plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRISPomyces-2BD was a gift from Min-Kyu Oh (Addgene plasmid # 234428 ; http://n2t.net/addgene:234428 ; RRID:Addgene_234428)

  • For your References section:

    Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite production. Kim DG, Gu B, Cha Y, Ha J, Lee Y, Kim G, Cho BK, Oh MK. Nat Commun. 2025 Jan 21;16(1):874. doi: 10.1038/s41467-025-56278-y. 10.1038/s41467-025-56278-y PubMed 39833194
Related items: