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pcDNA5-FRT/TO-APT2-LAMA-G97
(Plasmid #234426)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234426 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5 FRT/TO
  • Backbone manufacturer
    Thermo Fisher
  • Backbone size w/o insert (bp) 5100
  • Total vector size (bp) 6680
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    APT2 fused to the GFP enhancer nanobody containing dihydrofolate reductase inserted in the nanobody at position G97
  • Alt name
    LYPLA2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1593
  • Entrez Gene
    LYPLA2 (a.k.a. APT-2, APT2, DJ886K2.4)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-FRT/TO-APT2-LAMA-G97 was a gift from William Fuller (Addgene plasmid # 234426 ; http://n2t.net/addgene:234426 ; RRID:Addgene_234426)

  • For your References section:

    Nanobody-thioesterase chimeras to specifically target protein palmitoylation. Kuo CW, Gok C, Fulton H, Dickson-Murray E, Adu S, Gallen EK, Mary S, Robertson AD, Jordan F, Dunning E, Mullen W, Smith GL, Fuller W. Nat Commun. 2025 Feb 7;16(1):1445. doi: 10.1038/s41467-025-56716-x. 10.1038/s41467-025-56716-x PubMed 39920166
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