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Addgene

SARS2_HisSUMOTEV-nsp7/nsp8
(Plasmid #234405)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234405 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Vector type
    Bacterial Expression
  • Selectable markers
    Low Copy

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Nsp7_SARS2
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Promoter T7
  • Tag / Fusion Protein
    • His6-SUMO-TEV (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Nsp8_SARS2
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Tag / Fusion Protein
    • His6-SUMO-TEV (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SARS2_HisSUMOTEV-nsp7/nsp8 was a gift from Cheryl Arrowsmith (Addgene plasmid # 234405 ; http://n2t.net/addgene:234405 ; RRID:Addgene_234405)
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