L_EBOSU_MT:1713-2210_Nhis
(Plasmid
#234400)
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PurposeBacterial expression for structure determination. May not contain entire coding region of gene.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
Currently unavailable outside the U.S.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 234400 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a-LIC
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Vector typeBacterial Expression
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Selectable markersLow Copy
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameL_EBOSU
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SpeciesZaire ebolavirus
- Promoter T7
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Tag
/ Fusion Protein
- MGSSHHHHHHSSGLVPRGS (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
L_EBOSU_MT:1713-2210_Nhis was a gift from Cheryl Arrowsmith (Addgene plasmid # 234400 ; http://n2t.net/addgene:234400 ; RRID:Addgene_234400)
