VEEV_KP282671.1_NSP2_Hel_NHisCAvi
(Plasmid
#234398)
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PurposeBacterial expression for structure determination. May not contain entire coding region of gene.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234398 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNIC-Bio3
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Vector typeBacterial Expression
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Selectable markersLow Copy
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNsp2_VEEV
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SpeciesVenezuelan equine encephalitis virus
- Promoter T7
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Tags
/ Fusion Proteins
- MHHHHHHSSGVDLG (N terminal on insert)
- SSKGGYGLNDIFEAQKIEWHE (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
VEEV_KP282671.1_NSP2_Hel_NHisCAvi was a gift from Cheryl Arrowsmith (Addgene plasmid # 234398 ; http://n2t.net/addgene:234398 ; RRID:Addgene_234398)