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Addgene

Nbio-VP35:75-340_Cterm_HIS
(Plasmid #234391)

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This material is available to academics and nonprofits only. Currently unavailable outside the U.S.
Item Catalog # Description Quantity Price (USD)
Plasmid 234391 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBacMam2-DiEx-LIC
  • Vector type
    Baculovirus Expresssion
  • Selectable markers
    High Copy

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VP35_EBOZM
  • Species
    Zaire ebolavirus
  • Promoter CMV and P10
  • Tags / Fusion Proteins
    • MSGLNDIFEAQKIEWHEGSAGGSG (C terminal on insert)
    • SSGHHHHHH (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer pFBM-fwd (5'-caaaatgtcgtaacaactccgc-3')
  • 3′ sequencing primer pFBM-rev (5'- tagttaagaataccagtcaatctttcac-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Nbio-VP35:75-340_Cterm_HIS was a gift from Cheryl Arrowsmith (Addgene plasmid # 234391 ; http://n2t.net/addgene:234391 ; RRID:Addgene_234391)
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