SARS2nsp3_MacroD_EC
(Plasmid
#234389)
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PurposeBacterial expression for structure determination. May not contain entire coding region of gene.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 234389 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNIC-Bio3
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Vector typeBacterial Expression
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Selectable markersLow Copy
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNsp3_SARS2
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SpeciesSevere acute respiratory syndrome coronavirus 2
- Promoter T7
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Tag
/ Fusion Protein
- MHHHHHHSSGVDLGTENLYFQSM (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SARS2nsp3_MacroD_EC was a gift from Cheryl Arrowsmith (Addgene plasmid # 234389 ; http://n2t.net/addgene:234389 ; RRID:Addgene_234389)
