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Addgene

MERS_NSP13_BV_BIO
(Plasmid #234379)

Ordering

This material is available to academics and nonprofits only. Currently unavailable outside the U.S.
Item Catalog # Description Quantity Price (USD)
Plasmid 234379 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pFBD-BirA
  • Vector type
    Baculovirus Expresssion
  • Selectable markers
    High Copy

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nsp13_MERS
  • Species
    Middle East respiratory syndrome
  • Promoter Polyhedrin
  • Tags / Fusion Proteins
    • MSGLNDIFEAQKIEWHEGSAGGSG (N terminal on insert)
    • GGSGHHHHHH (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer tattccggattattcataccg
  • 3′ sequencing primer ctctacaaatgtggtatggc
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MERS_NSP13_BV_BIO was a gift from Cheryl Arrowsmith (Addgene plasmid # 234379 ; http://n2t.net/addgene:234379 ; RRID:Addgene_234379)