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Addgene

NSP13:5325-5925_BV
(Plasmid #234374)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234374 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFBOH-MHL
  • Backbone manufacturer
    Cheryl Arrowsmith, Addgene Plasmid #62304
  • Vector type
    Baculovirus Expresssion
  • Selectable markers
    High Copy

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nsp13_SARS2
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • MHHHHHHSSGRENLYFQG (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer pFBOH-fwd (CCGGATTATTCATACCGTCCCACCA)
  • 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NSP13:5325-5925_BV was a gift from Cheryl Arrowsmith (Addgene plasmid # 234374 ; http://n2t.net/addgene:234374 ; RRID:Addgene_234374)
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