MT-AvrXa10-FT
(Plasmid #234373)

• Purpose: T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-GFP-AvrXa10-GB1) with optimized activation domain (AvrXa10), targeting Arabidopsis FT locus
• Depositing Lab: Michael Smanski
• Publication: Zinselmeier et al Plant Biotechnol J. 2024 Nov;22(11):3202-3204. doi: 10.1111/pbi.14441. Epub 2024 Jul 26.

Backbone

• Vector backbone: pTRANS_220d
• Vector type: Plant Expression, Synthetic Biology
• Selectable markers: Kanamycin for plant selection

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: NbGP41-GFP-AvrXa10-GB1
• Species: Synthetic; Lama glama, X. oryzae
• Promoter: Arabidopsis Ubi10
• Tags / Fusion Proteins:
  • GFP
  • GB1

Cloning Information for Gene/Insert 1

• Cloning method: Golden Gate
• 5′ sequencing primer: none

Gene/Insert 2

• Gene/Insert name: dCas9-24XGP41
• Promoter: Arabidopsis Ubi10
• Tag / Fusion Protein:
  • GP41

Cloning Information for Gene/Insert 2

• Cloning method: Golden Gate
• 5′ sequencing primer: none

Gene/Insert 3

• Gene/Insert name: gRNAs targeting FT locus
• Species: A. thaliana (mustard weed), Synthetic
• Promoter: Arabidopsis U6

Cloning Information for Gene/Insert 3

• Cloning method: Golden Gate
• 5′ sequencing primer: none

Gene/Insert 4

• Gene/Insert name: RFP
• Species: Synthetic
• Promoter: Arabidopsis oleosin

Cloning Information for Gene/Insert 4

• Cloning method: Golden Gate
• 5′ sequencing primer: none

Resource Information

• Supplemental Documents:
  • mt-avrxa10-ft.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry