pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC
(Plasmid #234370)

• Purpose: T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs
• Depositing Lab: Michael Smanski
• Publication: Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):7083-7093. doi: 10.1093/nar/gkad458.

Backbone

• Vector backbone: pTRANS_220d
• Vector type: Plant Expression, Synthetic Biology
• Selectable markers: Kanamycin for plant selection

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: NbGP41-sfGFP-VP64-GB1
• Alt name: NbGP41-sfGFP-VP64
• Species: Synthetic; Lama glama, herpes simplex virus
• Promoter: Arabidopsis Ubi10
• Tags / Fusion Proteins:
  • sfGFP
  • GB1

Cloning Information for Gene/Insert 1

• Cloning method: Golden Gate
• 5′ sequencing primer: none

Gene/Insert 2

• Gene/Insert name: Luciferase
• Promoter: MTAP1 (synthetic)

Cloning Information for Gene/Insert 2

• Cloning method: Golden Gate
• 5′ sequencing primer: none

Gene/Insert 3

• Gene/Insert name: dCas9-13XGP41
• Promoter: Arabidopsis Ubi10
• Tag / Fusion Protein:
  • GP41

Cloning Information for Gene/Insert 3

• Cloning method: Golden Gate
• 5′ sequencing primer: none

Gene/Insert 4

• Gene/Insert name: gRNA 1-1 and gRNA 1-2
• Species: Synthetic
• Promoter: Arabidopsis U6

Cloning Information for Gene/Insert 4

• Cloning method: Golden Gate
• 5′ sequencing primer: none

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry