pCR4_miniFXN_7
(Plasmid #234360)

• Purpose: Mini FXN plasmid with a partial deletion of the 5' UTR and intron 1 in FXN. Does not contain GAA repeats.
• Depositing Lab: Marek Napierala
• Publication: Li et al Hum Gene Ther. 2020 Aug;31(15-16):839-851. doi: 10.1089/hum.2020.053. Epub 2020 Jul 13.

Backbone

• Vector backbone: pCR4
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 6710
• Vector type: Cloning

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FXN
• Alt name: frataxin
• Alt name: X25
• Alt name: FRDA GENE
• Species: H. sapiens (human)
• Insert Size (bp): 2224
• Mutation: There is a partial deletion in the 5'UTR of FXN, insert only contains 429 bp of the 5'UTR. There is a partial deletion in intron 1 of FXN, insert only contains 1165 bp of intron 1. 9272 bp of FXN intron 1 was deleted. First 1108 bp of intron 1 retained. Last 57 bp of intron 1 retained.
• Entrez Gene: FXN (a.k.a. CyaY, FA, FARR, FRDA, X25)
• Tag / Fusion Protein:
  • Flag Tag (C terminal on insert)

Resource Information

• Supplemental Documents:
  • HQT6CY_1_1-miniFXN7.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry