NP-11-64-1
(Plasmid
#234359)
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PurposeExpressing rubisco and prk in Δrpi
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234359 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep15A
- Backbone size w/o insert (bp) 4876
- Total vector size (bp) 6274
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameR.rubrum rubisco
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Alt nameRbcL and CbbM
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SpeciesRhodospirillum rubrum
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Insert Size (bp)1398
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GenBank IDWP_011390153
- Promoter Ptac
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer AATTGTGAGCGCTCACAAT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NP-11-64-1 was a gift from David Savage (Addgene plasmid # 234359 ; http://n2t.net/addgene:234359 ; RRID:Addgene_234359) -
For your References section:
A map of the rubisco biochemical landscape. Prywes N, Phillips NR, Oltrogge LM, Lindner S, Taylor-Kearney LJ, Tsai YC, de Pins B, Cowan AE, Chang HA, Wang RZ, Hall LN, Bellieny-Rabelo D, Nisonoff HM, Weissman RF, Flamholz AI, Ding D, Bhatt AY, Mueller-Cajar O, Shih PM, Milo R, Savage DF. Nature. 2025 Jan 22. doi: 10.1038/s41586-024-08455-0. 10.1038/s41586-024-08455-0 PubMed 39843747