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Addgene

NP-11-63
(Plasmid #234358)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234358 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28
  • Backbone size w/o insert (bp) 5571
  • Total vector size (bp) 6972
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For protein purification, use a DE3 strain (BL21-DE3). During expression, reduce temperature to 18C after IPTG induction
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    R.rubrum rubisco
  • Alt name
    RbcL and CbbM
  • Species
    Rhodospirillum rubrum
  • Insert Size (bp)
    1401
  • GenBank ID
    WP_011390153
  • Promoter T7
  • Tag / Fusion Protein
    • N-term SUMO tag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NP-11-63 was a gift from David Savage (Addgene plasmid # 234358 ; http://n2t.net/addgene:234358 ; RRID:Addgene_234358)

  • For your References section:

    A map of the rubisco biochemical landscape. Prywes N, Phillips NR, Oltrogge LM, Lindner S, Taylor-Kearney LJ, Tsai YC, de Pins B, Cowan AE, Chang HA, Wang RZ, Hall LN, Bellieny-Rabelo D, Nisonoff HM, Weissman RF, Flamholz AI, Ding D, Bhatt AY, Mueller-Cajar O, Shih PM, Milo R, Savage DF. Nature. 2025 Jan 22. doi: 10.1038/s41586-024-08455-0. 10.1038/s41586-024-08455-0 PubMed 39843747
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