pJEC779
(Plasmid
#234348)
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PurposeModular RENDR, GFP output, targets synthetic target
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 234348 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCloDF13
- Backbone size w/o insert (bp) 2125
- Total vector size (bp) 4229
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameModular RENDR, GFP output
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SpeciesSynthetic
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Insert Size (bp)2104
- Promoter PJ23119
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Na (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJEC779 was a gift from James Chappell (Addgene plasmid # 234348 ; http://n2t.net/addgene:234348 ; RRID:Addgene_234348) -
For your References section:
A split ribozyme that links detection of a native RNA to orthogonal protein outputs. Gambill L, Staubus A, Mo KW, Ameruoso A, Chappell J. Nat Commun. 2023 Feb 1;14(1):543. doi: 10.1038/s41467-023-36073-3. 10.1038/s41467-023-36073-3 PubMed 36725852