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Addgene

pAPH07
(Plasmid #234345)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234345 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMB1
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Type II dCas9 sgRNA
  • gRNA/shRNA sequence
    non-target (placeholder)
  • Promoter lacUV5

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.05.20.595006 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAPH07 was a gift from Jerome Fox (Addgene plasmid # 234345 ; http://n2t.net/addgene:234345 ; RRID:Addgene_234345)

  • For your References section:

    High-density CRISPRi screens reveal diverse routes to improved acclimation in cyanobacteria. Hren A, Lollini N, Carper DL, Abraham PE, Cameron JC, Fox JM, Eckert CA. Proc Natl Acad Sci U S A. 2025 Mar 25;122(12):e2412625122. doi: 10.1073/pnas.2412625122. Epub 2025 Mar 21. 10.1073/pnas.2412625122 PubMed 40117303
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