pAIP4_9C74
(Plasmid
#234238)
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PurposeHeterologous protein expression of variant of super folded GFP in Escherichia coli
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234238 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepET29b
- Backbone size w/o insert (bp) 5679
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name9C74
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Alt namesuper folder GFP
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Alt namevariant of super folded Green fluorescent protein
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SpeciesAequorea victoria
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Insert Size (bp)732
- Promoter T7
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Tag
/ Fusion Protein
- 10x HisTag-Smt3 (N terminal on backbone)
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer T7-promoter
- 3′ sequencing primer T7-Term (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bySynthetic DNA
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' cloning site: BsaI (destroyed), 3' cloning site: BsaI (destroyed)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAIP4_9C74 was a gift from Christopher Bahl (Addgene plasmid # 234238 ; http://n2t.net/addgene:234238 ; RRID:Addgene_234238)