pAIP4_6F2Q
(Plasmid #234224)

• Purpose: Heterologous protein expression of galectin-3 (Gal-3) (CBP 35) in Escherichia coli
• Depositing Lab: Christopher Bahl
• Publication: AI Proteins, Inc. plasmids (unpublished)

Backbone

• Vector backbone: pET29b
• Backbone size w/o insert (bp): 5679
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 6F2Q
• Alt name: LEG3_HUMAN
• Alt name: Galectin-3 (Gal-3) (35 kDa lectin) (Carbohydrate-binding protein 35) (CBP 35) (Galactose-specific lectin 3)
• Species: H. sapiens (human)
• Insert Size (bp): 414
• Entrez Gene: LGALS3 (a.k.a. CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2)
• Promoter: T7
• Tag / Fusion Protein:
  • 10x HisTag-Smt3 (N terminal on backbone)

Cloning Information

• Cloning method: Golden Gate
• 5′ sequencing primer: T7-promoter
• 3′ sequencing primer: T7-Term

Resource Information

• Supplemental Documents:
  • pAIP4_6F2Q.gb
• A portion of this plasmid was derived from a plasmid made by: Synthetic DNA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

5' cloning site: BsaI (destroyed), 3' cloning site: BsaI (destroyed). Galectin-3 (Gal-3) (35 kDa lectin) (Carbohydrate-binding protein 35) (CBP 35) (Galactose-specific lectin 3) (Galactoside-binding protein) (GALBP) (IgE-binding protein) (L-31) (Laminin-binding protein) (Lectin L-29) (Mac-2 antigen)