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Addgene

pAIP4_2NT3
(Plasmid #234208)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 234208 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pET29b
  • Backbone size w/o insert (bp) 5679
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    2NT3
  • Alt name
    O68522_MYXXA
  • Alt name
    Response regulator homolog
  • Species
    Myxococcus xanthus
  • Insert Size (bp)
    363
  • Promoter T7
  • Tag / Fusion Protein
    • 10x HisTag-Smt3 (N terminal on backbone)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Synthetic DNA

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' cloning site: BsaI (destroyed), 3' cloning site: BsaI (destroyed)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAIP4_2NT3 was a gift from Christopher Bahl (Addgene plasmid # 234208 ; http://n2t.net/addgene:234208 ; RRID:Addgene_234208)