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Addgene

pAIP4_3TOW
(Plasmid #234201)

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Item Catalog # Description Quantity Price (USD)
Plasmid 234201 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET29b
  • Backbone size w/o insert (bp) 5679
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3TOW
  • Alt name
    MB12B_HUMAN
  • Alt name
    Multivesicular body subunit 12B (ESCRT-I complex subunit MVB12B) (Protein FAM125B)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    438
  • Entrez Gene
    MVB12B (a.k.a. C9orf28, FAM125B)
  • Promoter T7
  • Tag / Fusion Protein
    • 10x HisTag-Smt3 (N terminal on backbone)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Synthetic DNA

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' cloning site: BsaI (destroyed), 3' cloning site: BsaI (destroyed)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAIP4_3TOW was a gift from Christopher Bahl (Addgene plasmid # 234201 ; http://n2t.net/addgene:234201 ; RRID:Addgene_234201)
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