pAIP4_2PPN
(Plasmid #234190)

• Purpose: Heterologous protein expression of peptidyl-prolyl cis-trans isomerase FKBP1A (PPIase FKBP1A) in Escherichia coli
• Depositing Lab: Christopher Bahl
• Publication: AI Proteins, Inc. plasmids (unpublished)

Backbone

• Vector backbone: pET29b
• Backbone size w/o insert (bp): 5679
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 2PPN
• Alt name: FKB1A_HUMAN
• Alt name: Peptidyl-prolyl cis-trans isomerase FKBP1A (PPIase FKBP1A) (EC 5.2.1.8)
• Species: H. sapiens (human)
• Insert Size (bp): 318
• Entrez Gene: FKBP1A (a.k.a. FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE)
• Promoter: T7
• Tag / Fusion Protein:
  • 10x HisTag-Smt3 (N terminal on backbone)

Cloning Information

• Cloning method: Golden Gate
• 5′ sequencing primer: T7-promoter
• 3′ sequencing primer: T7-Term

Resource Information

• Supplemental Documents:
  • pAIP4_2PPN.gb
• A portion of this plasmid was derived from a plasmid made by: Synthetic DNA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

5' cloning site: BsaI (destroyed), 3' cloning site: BsaI (destroyed). Peptidyl-prolyl cis-trans isomerase FKBP1A (PPIase FKBP1A) (EC 5.2.1.8) (12 kDa FK506-binding protein) (12 kDa FKBP) (FKBP-12) (Calstabin-1) (FK506-binding protein 1A) (FKBP-1A) (Immunophilin FKBP12) (Rotamase)