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Addgene

pAIP4_3ONH
(Plasmid #234179)

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Item Catalog # Description Quantity Price (USD)
Plasmid 234179 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET29b
  • Backbone size w/o insert (bp) 5679
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3ONH
  • Alt name
    UBA2_YEAST
  • Alt name
    Ubiquitin-activating enzyme E1-like (Polymerase-interacting protein 2) (SMT3-activating enzyme subunit 2)
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    333
  • Entrez Gene
    UBA2 (a.k.a. YDR390C, UAL1)
  • Promoter T7
  • Tag / Fusion Protein
    • 10x HisTag-Smt3 (N terminal on backbone)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Synthetic DNA

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' cloning site: BsaI (destroyed), 3' cloning site: BsaI (destroyed)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAIP4_3ONH was a gift from Christopher Bahl (Addgene plasmid # 234179 ; http://n2t.net/addgene:234179 ; RRID:Addgene_234179)
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