pAIP4_3K1H
(Plasmid
#234157)
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PurposeHeterologous protein expression of O25709_HELPY in Escherichia coli
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234157 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepET29b
- Backbone size w/o insert (bp) 5679
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3K1H
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Alt nameO25709_HELPY
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Alt nameUncharacterized protein
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SpeciesHelicobacter pylori 26695
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Insert Size (bp)345
- Promoter T7
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Tag
/ Fusion Protein
- 10x HisTag-Smt3 (N terminal on backbone)
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer T7-promoter
- 3′ sequencing primer T7-Term (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bySynthetic DNA
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' cloning site: BsaI (destroyed), 3' cloning site: BsaI (destroyed)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAIP4_3K1H was a gift from Christopher Bahl (Addgene plasmid # 234157 ; http://n2t.net/addgene:234157 ; RRID:Addgene_234157)