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Addgene

pAIP4_5IHW
(Plasmid #234148)

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Item Catalog # Description Quantity Price (USD)
Plasmid 234148 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET29b
  • Backbone size w/o insert (bp) 5679
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    5IHW
  • Alt name
    SDRE_STAAS
  • Alt name
    Serine-aspartate repeat-containing protein E
  • Species
    Staphylococcus aureus subsp. aureus MSSA476
  • Insert Size (bp)
    942
  • Promoter T7
  • Tag / Fusion Protein
    • 10x HisTag-Smt3 (N terminal on backbone)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Synthetic DNA

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' cloning site: BsaI (destroyed), 3' cloning site: BsaI (destroyed)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAIP4_5IHW was a gift from Christopher Bahl (Addgene plasmid # 234148 ; http://n2t.net/addgene:234148 ; RRID:Addgene_234148)
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