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Addgene

pKEK3235
(Plasmid #234107)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234107 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Fn Ori-p15a-Kan
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sfGFP
  • Species
    Synthetic; Aequorea Victoria
  • Mutation
    S30R/Y39N/N105T/Y145F/I171V/A206V
  • Promoter pJ23100-BsaI
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Golden Gate

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Universal primer overhangs for promoter swapping: Forward 5'- GGCTACGGTCTCGCGAC-3'; Reverse 5'- GGCTACGGTCTCGTAAT -3'

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKEK3235 was a gift from Karl Klose (Addgene plasmid # 234107 ; http://n2t.net/addgene:234107 ; RRID:Addgene_234107)
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