pKEK3229
(Plasmid
#234101)
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PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234101 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFn Ori-p15a-Kan
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameeforCP
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SpeciesEchinopara forskaliana
- Promoter pJ23100-BsaI
Cloning Information
- Cloning method Golden Gate
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Universal primer overhangs for promoter swapping: Forward 5'- GGCTACGGTCTCGCGAC-3'; Reverse 5'- GGCTACGGTCTCGTAAT -3'
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKEK3229 was a gift from Karl Klose (Addgene plasmid # 234101 ; http://n2t.net/addgene:234101 ; RRID:Addgene_234101)